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primary antibody against sod3  (Millipore)


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    Millipore primary antibody against sod3
    Primary Antibody Against Sod3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+against+sod3/anti+sod3/pm22542613-61-95-98
    Average 90 stars, based on 1 article reviews
    primary antibody against sod3 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Heterogeneity of endothelial cell phenotype within and amongst conduit vessels of the swine vasculature.
    Article Snippet: After protein separation, contents of the gel were transferred to a polyvinylidene difluoride membrane by the application of 34 V for 1 h, and this membrane was subsequently blocked with 5% non-fat milk in Tris-buffered saline–Tween (20 mM Tris–HCl, 137 mM NaCl and 0.1% Tween 20) at room temperature for 1 h. After 1 h incubation in non-fat milk, a primary antibody against eNOS (1:1000 dilution; BD transduction, Franklin Lakes, NJ, USA), HSP90 (1:1000 dilution; BD transduction), p67phox (1:1000 dilution; BD transduction), SOD1 (1:5000; Assay Designs, Farmingdale, NY, USA), SOD2 (1:2000 dilution; Assay Designs) or SOD3 (1:1000 dilution; Upstate/Millipore, Billerica, MA, USA) was applied overnight.



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    Primer sequence.

    Journal: Frontiers in Pharmacology

    Article Title: Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury

    doi: 10.3389/fphar.2021.626166

    Figure Lengend Snippet: Primer sequence.

    Article Snippet: Immunoblotting was performed using primary antibodies against ERRγ (Santa Cruz, sc-66883, 1:1,000), β-actin (Bioss, bs-0061R, 1:2,500), SOD1 (Proteintech, 67480-1-Ig, 1:5,000), SOD2 (Abclonal, A19576, 1:1,000), SOD3 (Abclonal, A6984, 1:1,000), cleaved caspase-3 (Cell Signaling Technology, 9,661, 1:1,000), and Bax (Cell Signaling Technology, 2,772, 1:1,000), followed by the incubation of peroxidase-conjugated goat anti-rabbit secondary antibody (Beyotime, A0208, 1:2,000).

    Techniques: Sequencing

    DY131 ameliorated oxidative stress in LPS-treated mice. (A,B) The levels of the antioxidant GSH and oxidative stress marker MDA in livers were measured using a commercial kit (n = 10 in each group). (C) mRNA expressions of SOD1, SOD2, and SOD3 were determined by qRT-PCR (n = 10 in each group). (D) Protein levels of SOD1, SOD2, and SOD3 were detected by Western blotting (n = 8 in each group). (E) Quantitative analyses of SOD1, SOD2, and SOD3 by densitometry (n = 8 in each group). (F) Representative images of DHE staining of liver tissues in different groups (magnification ×200, scale bar: 50 μm, n = 3 in each group). (G) Quantification of the mean fluorescence intensity of DHE was analyzed by ImageJ software. Data were presented as means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 vs. the indicated group. NS, no significance.

    Journal: Frontiers in Pharmacology

    Article Title: Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury

    doi: 10.3389/fphar.2021.626166

    Figure Lengend Snippet: DY131 ameliorated oxidative stress in LPS-treated mice. (A,B) The levels of the antioxidant GSH and oxidative stress marker MDA in livers were measured using a commercial kit (n = 10 in each group). (C) mRNA expressions of SOD1, SOD2, and SOD3 were determined by qRT-PCR (n = 10 in each group). (D) Protein levels of SOD1, SOD2, and SOD3 were detected by Western blotting (n = 8 in each group). (E) Quantitative analyses of SOD1, SOD2, and SOD3 by densitometry (n = 8 in each group). (F) Representative images of DHE staining of liver tissues in different groups (magnification ×200, scale bar: 50 μm, n = 3 in each group). (G) Quantification of the mean fluorescence intensity of DHE was analyzed by ImageJ software. Data were presented as means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 vs. the indicated group. NS, no significance.

    Article Snippet: Immunoblotting was performed using primary antibodies against ERRγ (Santa Cruz, sc-66883, 1:1,000), β-actin (Bioss, bs-0061R, 1:2,500), SOD1 (Proteintech, 67480-1-Ig, 1:5,000), SOD2 (Abclonal, A19576, 1:1,000), SOD3 (Abclonal, A6984, 1:1,000), cleaved caspase-3 (Cell Signaling Technology, 9,661, 1:1,000), and Bax (Cell Signaling Technology, 2,772, 1:1,000), followed by the incubation of peroxidase-conjugated goat anti-rabbit secondary antibody (Beyotime, A0208, 1:2,000).

    Techniques: Marker, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Software